Lung Function Measurement in Lactating Dams

The EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich) (0–25 mg/mL, 10% puff for 10 s) was used to determine lung function of dams on day 21 of lactation (day 42 of the experiment) as performed previously (Verheijden et al., 2014 (link)). Mice were anesthetized by intraperitoneal injection of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, Netherlands; 0.4 mg/kg) mixture. Data are expressed as average lung resistance (RL) in cm H2O/mL × sec^–1.

Free full text: Click here

Janbazacyabar H., van Daal M., Leusink-Muis T., van Ark I., Garssen J., Folkerts G., van Bergenhenegouwen J, & Braber S. (2021). The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?. Frontiers in Cell and Developmental Biology, 9, 680902.

Publication 2021

EMKA invasive measurement of dynamic resistance acetyl-β-methyl-choline chloride ketamine medetomidine

Choline chloride Intraperitoneal injection Ketamine Lactation Lung Lung function Medetomidine Methacholine Mice

Corresponding Organization : Utrecht University

Other organizations : Danone (Netherlands), Nutricia Research (Netherlands)

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich) (0–25 mg/mL, 10% puff for 10 s)

dependent variables

Lung function of dams on day 21 of lactation (day 42 of the experiment)

control variables

Anesthesia by intraperitoneal injection of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, Netherlands; 0.4 mg/kg) mixture

controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!