Western Blot Protein Detection Protocol

Western Blot experiments were performed as previously described (Troschel et al. 2020 (link)). About 10⁷ cells were trypsinized and lysed. Subsequently, 30 µg of whole protein was electrophoresed and transferred to nitrocellulose and thus used for immuno-detection. Antibody binding was visualized using ECL peroxidase blotting substrate (Thermo Fisher Scientific). Then, quantification of luminescence was performed with a Fusion SL System (Peqlab, Erlangen, Germany). Primary and secondary antibody details are shown in Supplementary Table S3.

Free full text: Click here

Troschel F.M., Palenta H., Borrmann K., Heshe K., Hua S.H., Yip G.W., Kiesel L., Eich H.T., Götte M, & Greve B. (2021). Knockdown of the prognostic cancer stem cell marker Musashi-1 decreases radio-resistance while enhancing apoptosis in hormone receptor-positive breast cancer cells via p21WAF1/CIP1. Journal of Cancer Research and Clinical Oncology, 147(11), 3299-3312.

Publication 2021

Fusion SL System

Antibody Cells Luminescence Nitrocellulose Peroxidase Protein Western blot

Corresponding Organization : University Hospital Münster

Other organizations : National University of Singapore

Top 5 similar protocols

Variable analysis

independent variables

Cell treatment (type of cells used)

dependent variables

Protein expression levels (detected by Western blot analysis)

control variables

Cell number (10^7 cells)
Amount of protein loaded (30 µg whole protein)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!