Quantitative Protein Expression Analysis

Western blot was performed as previously described (31 (link)). Briefly, total proteins of tumor tissues were isolated with RIPA buffer (#P0013C, Beyotime) and the concentrations of which were measured using a BCA detecting kit (#P0012, Beyotime). The lysates were fractionated by SDS PAGE and transferred to PVDF membranes. Primary and secondary antibodies were used to detect the targets on the membranes. The primary antibodies used were: anti-OXCT1 (#12175-1-AP, Proteintech), anti-HMGCS2 (#ab137043, Abcam), anti-ENO2 (#10149-1-AP, Proteintech), anti-PKM2 (#15822-1-AP, Proteintech), anti-HK2 (#22029-1-AP, Proteintech), anti-β-actin (#4970s, CST). The quantitative analysis was performed using Photoshop.