Twenty female 8- to 10-week-old NOD.CB17- Prkdcscid/NCrHsd mice (NOD-SCID; Envigo, Huntingdon, UK) were injected intratibially with 2×105 RPMI-8226 cells suspended in phosphate-buffered saline. Mice were treated with the anti-JAM-A monoclonal antibody (Sigma-Aldrich, St. Louis, MO, USA, mouse monoclonal clone J10.4) recognizing the distal membrane extracellular domain of JAM-A. Twenty female 6- to 8-week-old NOD-SCID mice were injected subcutaneously (s.c.), into the right flank, with 1×107 RPMI-8226 cells suspended in 200 mL RPMI-1640 medium and 200 mL MatrigelTM as described previously16 (link) and detailed in the Online Supplementary Methods.
Partial Protocol Preview
This section provides a glimpse into the protocol. The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Solimando A.G., Da Viá M.C., Leone P., Borrelli P., Croci G.A., Tabares P., Brandl A., Di Lernia G., Bianchi F.P., Tafuri S., Steinbrunn T., Balduini A., Melaccio A., De Summa S., Argentiero A., Rauert-Wunderlich H., Frassanito M.A., Ditonno P., Henke E., Klapper W., Ria R., Terragna C., Rasche L., Rosenwald A., Kortüm K.M., Cavo M., Ribatti D., Racanelli V., Einsele H., Vacca A, & Beilhack A. (2020). Halting the vicious cycle within the multiple myeloma ecosystem: blocking JAM-A on bone marrow endothelial cells restores angiogenic homeostasis and suppresses tumor progression. Haematologica, 106(7), 1943-1956.
Cells Clone Female Jam a Membrane Monoclonal antibody Mouse Nod mice Phosphate SalineScid mice
Corresponding Organization :
Other organizations :
Universitätsklinikum Würzburg, University of Bari Aldo Moro, Istituto Tumori Bari, Istituti di Ricovero e Cura a Carattere Scientifico, University of Pavia, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, University of Milan, University Hospital Schleswig-Holstein, University of Lübeck, Kiel University, Tufts University, Tumori Foundation, University of Würzburg, Istituto Oncologico Romagnolo
Mouse strain (NOD.CB17-Prkdcscid/NCrHsd mice, also known as NOD-SCID)
Mouse age (8-10 weeks old, 6-8 weeks old)
Cell line (RPMI-8226)
Cell suspension (phosphate-buffered saline, RPMI-1640 medium and Matrigel)
controls
Positive control: None mentioned
Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
