3'-RACE for lncTAM34a Identification

3′-RACE was performed as described as previously in Ref. 8 (link). Briefly, U2OS cell RNA was polyA-tailed using yeast polyA polymerase (ThermoFisher Scientific, Cat# 74225Z25KU) after which cDNA was synthesized using oligo(dT) primers. Nested-PCR was performed first using a forward primer in lncTAM34a exon 1 and a tailed oligo(dT) primer followed by a second PCR using an alternate lncTAM34a exon 1 primer and a reverse primer binding to the tail of the previously used oligo(dT) primer. PCR products were gel purified and cloned the Strata Clone Kit (Agilent Technologies, Santa Clara, CA, USA, Cat# 240205), and sequenced.