Flow Cytometry Analysis of MCF10DCIS Cell Subpopulations

The detailed procedure was reported previously [24 (link)]. MCF10DCIS cells isolated from mammospheres were stained with antibodies against CD44-APC, Clone G44–26 from BD Biosciences (Cat# 559942, RRID: AB_398683) and CD10-PE from Thermo Fisher Scientific (Cat# 12–0106-41, RRID: AB_10714985). The stained MCF10DCIS cells were analyzed by flow cytometry using an FC500 Analyzer (Beckman Coulter) to determine the percentage of 4 different CD44⁻/CD10⁺, CD44⁺/CD10⁺, CD44⁻/CD10⁻ and CD44⁺/CD10⁻subpopulations.

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Shan N.L., Minden A., Furmanski P., Bak M.J., Cai L., Wernyj R., Sargsyan D., Cheng D., Wu R., Kuo H.C., Li S.N., Fang M., Maehr H., Kong A.N, & Suh N. (2020). Analysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma In Situ by Vitamin D Compounds. Cancer prevention research (Philadelphia, Pa.), 13(8), 673-686.

Publication 2020

Clone G44–26 FC500 Analyzer

Antibodies Cd44 Cells Clone Flow cytometry Subpopulations

Corresponding Organization : Rutgers, The State University of New Jersey

Other organizations : Environmental and Occupational Health Sciences Institute

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Variable analysis

independent variables

Antibodies against CD44-APC, Clone G44–26 from BD Biosciences (Cat# 559942, RRID: AB_398683)
Antibodies against CD10-PE from Thermo Fisher Scientific (Cat# 12–0106-41, RRID: AB_10714985)

dependent variables

Percentage of 4 different CD44-/CD10+, CD44+/CD10+, CD44-/CD10- and CD44+/CD10- subpopulations in MCF10DCIS cells

control variables

MCF10DCIS cells isolated from mammospheres

controls

No positive or negative controls were explicitly mentioned in the input.

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