The responsive microswimmers were prepared using sequential capillarity-assisted particle assembly (sCAPA). The basic principles of sCAPA are described by Ni et al. (22 (link)). Here, we used an adapted version of sCAPA, which uses masters prepared with direct writing via two-photon nanolithography, as opposed to Si masters made with conventional lithography methods. Briefly, negative masters were prepared with two-photon lithography (Nanoscribe Photonic Professional GT2) using a 63x oil-immersion objective and IP-Dip solution set (37 ). The masters contained 6 smaller areas with 10,000 “traps" each, and one area was used to prepare the particles for one experiment. After printing and developing under standard conditions, these masters were postcured for 2 h under a UV-lamp and coated with a perfluoro silane using chemical vapor deposition. PDMS (Sylgard 184) was used to make templates from these masters. sCAPA was performed as described in the following paper (38 (link)). The PS particles were deposited from a 0.035% Triton X-45 and 3.5 mM SDS solution in Milli-Q water. The microgels were from a 0.04% Triton X-45 in 1 mM HEPES pH 7.4 or 0.025% Triton X-45 in 1 mM MES pH 4.5 for green- and red-core microgels, respectively. All depositions were carried out at 25 °C with a deposition rate of 3 µm s−1.