Immunohistochemical Profiling of Prostate Cancer

Paraffin-embedded sections of prostate cancer samples were deparaffinized, rehydrated, and microwaved for 20 min in Tris/EDTA buffer or citric acid buffer to retrieve antigens. Staining was carried out using primary antibodies against the following proteins: Gankyrin (ab182576, Abcam), HMGB1 (ab18256, Abcam), CD68 (M0876, Dako), STAT3 (ab32500, Abcam), p-STAT3 (ab76315, Abcam), and NONO (11058, Proteintech). Samples were scored semiquantitatively according to the percentage of staining intensity and positive cells using the H score (range 0–300), as described previously.^{20 (link)} All the immunohistochemistry assays were repeated three times.

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Peng G., Wang C., Wang H., Qu M., Dong K., Yu Y., Jiang Y., Gan S, & Gao X. (2023). Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance. Oncoimmunology, 12(1), 2173422.

Publication 2023

ab182576 ab18256 M0876 ab32500 ab76315

Antibodies Antigens Assays Buffer Cancer of prostate Cells Citric acid Edta Embedded paraffin Gankyrin Hmgb1 Immunohistochemistry Proteins Stat3 Tris buffer

Corresponding Organization : Changhai Hospital

Other organizations : General Hospital of Guangzhou Military Command, Wuhan General Hospital of Guangzhou

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Variable analysis

independent variables

Tris/EDTA buffer or citric acid buffer used for antigen retrieval

dependent variables

Staining intensity and percentage of positive cells for the following proteins: Gankyrin, HMGB1, CD68, STAT3, p-STAT3, and NONO

control variables

Prostate cancer samples
Deparaffinization and rehydration of samples
Microwave treatment for 20 minutes for antigen retrieval
Primary antibodies used for staining

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