Inhibiting Fucosylated Structures Synthesis

To inhibit the synthesis of fucosylated structures, a competitive inhibitor of fucosyltransferases, the 2F-Peracetyl-Fucose (Merck) was used. This inhibitor enters the cell by passive diffusion and is metabolized into a GDP-fucose analog (2F-fucose) to form an inhibitory substrate of fucosyltransferases^{60 (link)}. Cells were seeded in 96-well plates or 25 cm² flasks and treated with 500 µM 2F-Peracetyl-Fucose (Merck) solubilized in DMSO in the adequate medium for 3 days, replacing the medium with fresh drug daily. Negative controls were performed by adding the DMSO vehicle only to the culture medium. Afterwards, treated cells were phenotyped by flow cytometry or infected by RVs.