Culturing Human Ovarian Cancer Cell Lines

SKOV3, CAOV3, OVCAR3 and A2780 human ovarian cancer cell lines were purchased from American Type Culture Collection. OV75 primary ovarian cancer cells were provided by Dr Jocelyn Reader and Dr Dana Roque, who were the original suppliers (Division of Gynecologic Oncology, University of Maryland School of Medicine, Baltimore, MD, USA). OV75 cells are primary ovarian cancer cells available for research labs from the University of Maryland. The SKOV3, CAOV3 and A2780 cells were cultured in DMEM (Corning, Inc.) supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA) and 1% penicillin/streptomycin (PS). OVCAR3 cells were cultured in RPMI 1640 medium (Corning, Inc.) with 10% FBS and 1% PS. OV75 cells were cultured in HOSE Media [1:1 mixture of MCDB 105 (Sigma-Aldrich; Merck KGaA) and Medium 199 (Thermo Fisher Scientific, Inc.)], 10% FBS, 1% L-glutamine, 1% non-essential amino acids, 1% PS, sodium bicarbonate at pH 7.4) as described previously (17 (link)). All cell lines were grown in a humidified 37°C incubator with 5% CO₂/95% air, and the medium was replaced twice a week.