Genomic DNA of the whole blood was extracted using the TIANamp Blood Genomic DNA Purification kit (Tiangen inc. Beijing, China). The criteria of DNA quality control were DNA concentration should be larger than 50 ng/μL, the ratio of OD260/OD280 in the range of 1.7–1.9 and the ratio of OD260/OD230 in the range of 1.5–2.1.
The cows were genotyped using Illumina Bovine SNP50 BeadChip [25 (link)]. The genotypes were edited according to the criteria: (1) call rate > = 90 %; (2) SNPs did not deviated extremely from Hardy-Weinberg equilibrium (P >10−6); (3) minor allele frequency > = 3 %). After quality control, a total of 43,885 SNPs were available for MMRA. Distribution of SNPs on each chromosome after quality control and the average distances between adjacent SNPs are shown in Additional file1 : Table S1.
The cows were genotyped using Illumina Bovine SNP50 BeadChip [25 (link)]. The genotypes were edited according to the criteria: (1) call rate > = 90 %; (2) SNPs did not deviated extremely from Hardy-Weinberg equilibrium (P >10−6); (3) minor allele frequency > = 3 %). After quality control, a total of 43,885 SNPs were available for MMRA. Distribution of SNPs on each chromosome after quality control and the average distances between adjacent SNPs are shown in Additional file
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