The concentrations of IL-1β and IL-6 in the sera of C57BL/6 mice and cell culture media from RAW264.7 cells were measured by ELISA according to the manufacturer’s instructions (ELISA kits for IL-1β: eBioscience, Frankfurt, Germany; ELISA kits for IL-6: R&D Systems, Minneapolis, MN, U.S.A.). All assays were performed in duplicate, as previously reported29 (link).
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