The release of gallic acid from the mesoporous silica was studied in two simulated biological fluids [31 (link)]: simulated gastric fluid (SGF) with pH = 1.2 and simulated intestinal fluid (SIF) with pH = 6.8, considering the intention to use the materials for oral administration or in the form of suppositories. Release studies were performed at a constant temperature of 37 ± 1 °C and a magnetic stirring of 270 rpm. Working conditions are presented in Table 2 .
The amount of active substance released was determined using liquid chromatography using an Agilent liquid chromatograph with DAD-type UV-Vis detector. In order to determine the amount of active substance in the samples, calibration curves were made for gallic acid in the two simulated biological fluids (SGF and SIF) in areas of adequate concentrations relative to the theoretical concentrations of gallic acid.
The amount of active substance released was determined using liquid chromatography using an Agilent liquid chromatograph with DAD-type UV-Vis detector. In order to determine the amount of active substance in the samples, calibration curves were made for gallic acid in the two simulated biological fluids (SGF and SIF) in areas of adequate concentrations relative to the theoretical concentrations of gallic acid.
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