RAPD Amplification and Gel Electrophoresis

The RAPD amplification mixture and cycling conditions were described elsewhere (Lambiase et al., 2006 (link)). The primer used was the 270 (5′-TGCGCGCGGG-3′). RAPD products were separated by electrophoresis in 1.5% agarose gel (Invitrogen Corporation, CA). Molecular size markers (Invitrogen Corporation) and negative control were included in all gels. Gels were stained with EtBr (at 0.5 μM) (Invitrogen Corporation) and captured with a DigiDoc-It (UVP) photographic system.

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Trancassini M., Iebba V., Citerà N., Tuccio V., Magni A., Varesi P., De Biase R.V., Totino V., Santangelo F., Gagliardi A, & Schippa S. (2014). Outbreak of Achromobacter xylosoxidans in an Italian Cystic fibrosis center: genome variability, biofilm production, antibiotic resistance, and motility in isolated strains. Frontiers in Microbiology, 5, 138.

Publication 2014

agarose gel Molecular size markers DigiDoc-It

Agarose Electrophoresis Etbr Gels Molecular markers Primer Rapd

Corresponding Organization :

Other organizations : Sapienza University of Rome, Cystic Fibrosis Research Foundation

Top 5 similar protocols

Variable analysis

independent variables

Primer used (270 (5′-TGCGCGCGGG-3′))

dependent variables

RAPD products

control variables

RAPD amplification mixture and cycling conditions
Molecular size markers (Invitrogen Corporation)
Negative control

controls

Negative control

Annotations

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