Quantifying Lung Barrier Integrity and Cytotoxicity

The albumin content in BAL was quantified as an indicator of the increased permeability of the bronchoalveolar capillary barrier [21 (link)]. In addition, lactate dehydrogenase (LDH) activity was quantified as an indicator of general cytotoxicity [22 (link)]. Albumin content was determined colorimetrically using a diagnostic kit (Wiener Lab, Buenos Aires, Argentina) that is based on the binding of albumin to bromocresol green. LDH activity, expressed as units per litre of BAL fluid, was determined using Wiener’s reagents and procedures (Wiener Lab), which measure the formation of the reduced form of nicotinamide adenine dinucleotide (NAD).

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Raya Tonetti F., Tomokiyo M., Ortiz Moyano R., Quilodrán-Vega S., Yamamuro H., Kanmani P., Melnikov V., Kurata S., Kitazawa H, & Villena J. (2021). The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae. Microorganisms, 9(6), 1324.

Publication 2021

Wiener’s reagents and procedures

Albumin Bromocresol green Capillary permeability Cytotoxicity Diagnostic Lactate dehydrogenase Reduced nicotinamide adenine dinucleotide

Corresponding Organization : Tohoku University

Other organizations : University of Concepción, Gabrichevsky Institute of Epidemiology and Microbiology

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Variable analysis

independent variables

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dependent variables

Albumin content in BAL
Lactate dehydrogenase (LDH) activity in BAL fluid

control variables

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controls

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