Synthesis and Purification of Inositol Phosphates

Adenophostin A34 , Ins(1,3,4,5)P₄ (ref. 63 ) scyllo-InsP₅ (ref. 31 (link)), Bz(1,2,3,4)P₄ (ref. 32 (link)), 1-PA-InsP₅ (ref. 36 (link)), 2-OH-5-PA-InsP₄ (ref. 37 ), 2-FAM-Ins(1,3,4,5,6)P₅ (ref. 38 (link)) were synthesized as previously reported. The identities and purities of these compounds were confirmed by ¹H and ³¹P NMR spectroscopy. Ins(1,3,5,6)P₄, Ins(3,4,5,6)P₄, Ins(1,3,4,5,6)P₅, Ins(1,4,5)P₃, Ins(1,4,6)P₃, Ins(1,5,6)P₃, were purchased from Cayman Chemical Company. InsP₆ was purchased from Sigma. Details of the new syntheses of Ins(1,4,5,6)P₄, Ins(4,5,6)P₃, Ins(1,3,4,6)P₄, 2-O-Bn-Ins(1,4,5,6)P₄ and 2,3-di-O-Bn-Ins(1,4,5,6)P₄ are given in the Supplementary Methods.