Characterizing Murine Type 2 Immunity

IL5Tg (5 (link)), Arg1^YFP (42 (link)), Il5^Red5Cre (Red5) (10 (link)), ILC2-deleted (ILC2^del) (10 (link)); STAT6^–/– (43 (link)), Smart13 (35 (link)), IL4Rα^–/– (44 (link)), and IL4^–/–/IL13^–/– (45 (link)) mice have been described. Mice were derived or backcrossed on a C57BL/6J background for more than 10 generations, and housed under specific pathogen–free conditions with 12-hr light/day cycles and ad libitum access to food and water. Experiments were performed on age- and sex-matched male and female mice between 8 weeks and 16 weeks of age, except for the eotaxin experiments, in which mice were 6 months of age. IL-33 (BioLegend or R&D), TSLP (eBioscience), or IL-25 (R&D) were administered intranasally at 0.5 μg per dose, 3 times a week for 2 weeks, and mice were analyzed 1 day after the final dose. For intravascular labeling experiments, mice were injected intravenously with 3 μg of anti-mouse CD45 Ab (clone 30-F11) 3 minutes prior to sacrifice. Eotaxin was measured from serum using the Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D).

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Kotas M.E., Dion J., Van Dyken S., Ricardo-Gonzalez R.R., Danel C.J., Taillé C., Mouthon L., Locksley R.M, & Terrier B. (2021). A role for IL-33–activated ILC2s in eosinophilic vasculitis. JCI Insight, 6(12), e143366.

Publication 2021

IL-33 IL-25 Mouse CCL11/Eotaxin Quantikine ELISA Kit

Clone Elisa Eotaxin Female Food Il 25 Il 33 Il13 Il4rα Mouse Serum Sex male Specific pathogen free Stat6

Corresponding Organization : Assistance Publique – Hôpitaux de Paris

Other organizations : University of California System, University of California, San Francisco, Washington University in St. Louis, Hôpital Bichat-Claude-Bernard, Howard Hughes Medical Institute

Top 5 similar protocols

Variable analysis

independent variables

IL5Tg (5 (link))
Arg1^YFP (42 (link))
Il5^Red5Cre (Red5) (10 (link))
ILC2-deleted (ILC2^del) (10 (link))
STAT6^–/– (43 (link))
Smart13 (35 (link))
IL4Rα^–/– (44 (link))
IL4^–/–/IL13^–/– (45 (link))
IL-33 (BioLegend or R&D), TSLP (eBioscience), or IL-25 (R&D) administered intranasally at 0.5 μg per dose, 3 times a week for 2 weeks

dependent variables

Eotaxin measured from serum using the Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D)

control variables

Mice were derived or backcrossed on a C57BL/6J background for more than 10 generations
Mice were housed under specific pathogen–free conditions with 12-hr light/day cycles and ad libitum access to food and water
Experiments were performed on age- and sex-matched male and female mice between 8 weeks and 16 weeks of age, except for the eotaxin experiments, in which mice were 6 months of age
For intravascular labeling experiments, mice were injected intravenously with 3 μg of anti-mouse CD45 Ab (clone 30-F11) 3 minutes prior to sacrifice

positive controls

None specified

negative controls

None specified

Annotations

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