Transfected cells were scored based on the αSyn inclusions pattern and classified into: cells without inclusions, less than ten inclusions (<10 inclusions), and more than ten inclusions (≥10 inclusions), as described [61 (link)]. The total number of transfected cells was expressed in percentage, as the average from three independent experiments. The lactate dehydrogenase (LDH) cytotoxicity assay (Roche Diagnostics) was performed according to the manufacturer’s instructions. Growth media from cells were applied in triplicates in a 96-well plate, in a ratio 1:1 with the reaction mixture. The measurements were performed in a TECAN Infinite 200 Pro plate reader at 490 nm. The percentage of toxicity was calculated as indicated by the manufacturer.
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