Protocol detail

Histological Evaluation of Colitis

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Paraffin-embedded sections were cut at 4 μm thickness, stained with hematoxylin and eosin (H & E), and examined by a pathologist blinded to the experimental groups. The extent of colitis was scored according to the protocols described previously [40 (link)]. Immunohistochemical analysis was performed as we described [20 (link)], using the following primary antibodies: anti-CD68, anti-MR (all from Abcam, Cambridge, UK), anti-CD31 and anti-MMP2 (Santa Cruz, Dallas, USA).

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Wu T., Dai Y., Wang W., Teng G., Jiao H., Shuai X., Zhang R., Zhao P, & Qiao L. (2016). Macrophage targeting contributes to the inhibitory effects of embelin on colitis-associated cancer. Oncotarget, 7(15), 19548-19558.

Publication 2016

anti-CD68 anti-MR anti-CD31 anti-MMP2

Antibodies Colitis Eosin Mmp2 Paraffin Pathologist

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Variable analysis

independent variables

Experimental groups

dependent variables

Extent of colitis

control variables

Thickness of paraffin-embedded sections (4 μm)
Staining with hematoxylin and eosin (H&E)
Pathologist blinding to experimental groups

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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