Biomarker Analysis in Parkinson's Disease

Biomarker analyses have been previously described and based on the PPMI biologics manual (Marek et al., 2018 (link)). CSF was collected by standardized lumbar puncture procedures. Measurements of Aβ₄₂ and t-tau were analyzed by using the xMAP-Luminex platform with INNOBIA AlzBio3 immunoassay kit–based reagents (Fujirebio-Innogenetics, Ghent, Belgium) at Penn (Kang et al., 2013 (link)). Additionally, CSF total α-syn levels were measured by BioLegend (San Diego, CA, United States) by means of a commercially accessible and previously described sandwich immunoassay. Serum NFL was quantified by the Simoa Human NF-light Advantage Kit (Quanterix, Lexington, MA, United States) using the Single Molecule Array technology in a fully automated SIMOA HD-1 analyzer. Biochemical analyses of uric acid have been carried out in Covance laboratories in a uniform fashion, as per the study protocol.

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Dou K., Ma J., Zhang X., Shi W., Tao M, & Xie A. (2022). Multi-predictor modeling for predicting early Parkinson’s disease and non-motor symptoms progression. Frontiers in Aging Neuroscience, 14, 977985.

Publication 2022

xMAP-Luminex platform INNOBIA AlzBio3 immunoassay kit Simoa Human NF-light Advantage Kit

Biologics Biomarker Human Immunoassay Light Lumbar puncture procedures Serum Uric acid

Corresponding Organization : Affiliated Hospital of Qingdao University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Aβ42 levels in CSF
T-tau levels in CSF
Total α-syn levels in CSF
Serum NFL levels

control variables

Lumbar puncture procedure for CSF collection was standardized

controls

No positive or negative controls were explicitly mentioned in the provided information.

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