Synuclein and Cofilin Expression Vectors

pCMV6-Entry vectors encoding FLAG-tagged human α-synuclein (RC210606) and β-synuclein (RC215165), pCMV6-AC-GFP vectors encoding TurboGFP-tagged human α- (RG210606) and β-synuclein (RG215165), pLenti-C-mGFP-P2A-Puro lentiviral control particles (PS100093V) and pLenti-C-mGFP-P2A-Puro encoding mGFP-tagged human cofilin (RC203585L4V) were from Origene. The mito-Keima construct (mt/mKeima/pIND(SP1)) was a gift from Dr. A. Miyawaki (RIKEN Brain Science Institute, Japan). The Keima construct (mKeima-Red-N1) was a gift from Dr M. Davidson (Addgene, 54597). The pEGFP-C1 vector was a gift from Dr P. Vangheluwe (KU Leuven). Cloning of Keima and mito-Keima cDNA into lentivirus and production of lentiviral particles were described before^{17 (link)}.

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Kinnart I., Manders L., Heyninck T., Imberechts D., Praschberger R., Schoovaerts N., Verfaillie C., Verstreken P, & Vandenberghe W. (2024). Elevated α-synuclein levels inhibit mitophagic flux. NPJ Parkinson's Disease, 10, 80.

Publication 2024

pLenti-C-mGFP-P2A-Puro

Corresponding Organization : KU Leuven

Other organizations : VIB-KU Leuven Center for Brain & Disease Research

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Variable analysis

independent variables

Overexpression of FLAG-tagged human α-synuclein (RC210606) and β-synuclein (RC215165)
Overexpression of TurboGFP-tagged human α-synuclein (RG210606) and β-synuclein (RG215165)
Overexpression of mGFP-tagged human cofilin (RC203585L4V)

dependent variables

Not explicitly mentioned

control variables

PLenti-C-mGFP-P2A-Puro lentiviral control particles (PS100093V)

controls

Positive control: pLenti-C-mGFP-P2A-Puro lentiviral control particles (PS100093V)
Negative control: Not explicitly mentioned

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