Synthesis and Characterization of Fluorophenyl-Imidazole

The 2-fluorophenyl-imidazole used in this study was synthesized and provided by Dr. Thais Rossa, supervised by Professor Dr. Marcus Mandolesi Sá of the Department of Chemistry at the Universidade Federal de Santa Catarina (UFSC). The substituted fluorophenyl-imidazole (Figure 1) was synthesized by a reaction involving an azirine, a primary amine and an aldehyde [18 (link)]. The imidazole tested has a purity greater than 99% and was dissolved in 1% dimethyl sulfoxide (DMSO), following the predefined concentrations. After that, fluorophenyl-imidazole was aliquoted and stored at −80°C until the experiments, when it was properly dissolved in a cell culture medium.