All NMR experiments were performed at 37 °C on a Bruker AVIII-500 NMR spectrometer (Bruker Corporation, Germany). The samples were prepared in D2O. As an external reference, 0.05% 3-(trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP) was used to calibrate the NMR chemical shifts. The spectra were analyzed with reference to the internal signal of TSP-d 4 protons at 0 ppm. Resonance assignments for Mog-V were made according to 2D 1H-1H COSY, 1H-13C HMBC, and 1H-13C HMQC NMR spectra and the reference data.24 (link) The chemical shifts of protons in Mog-V were recorded as a function of the concentrations (1, 2, 3, 4, 5, 8, 10, 15, 20, 30 and 50 mg/mL).
The 2D 1H-1H NOESY spectra for 1.0 and 50 mg/mL Mog-V solutions were measured to check the molecular conformation of Mog-V in the aqueous solution. Under similar conditions, 1H NMR spectra and 2D 1H-1H NOESY spectra of CUR/Mog-V SDP solutions (concentration equivalent to 50 mg/mL of Mog-V) were also recorded to validate the interaction positions between CUR and Mog-V molecules. The peaks of CUR were assigned based on prior reports.25 (link)
The 2D 1H-1H NOESY spectra for 1.0 and 50 mg/mL Mog-V solutions were measured to check the molecular conformation of Mog-V in the aqueous solution. Under similar conditions, 1H NMR spectra and 2D 1H-1H NOESY spectra of CUR/Mog-V SDP solutions (concentration equivalent to 50 mg/mL of Mog-V) were also recorded to validate the interaction positions between CUR and Mog-V molecules. The peaks of CUR were assigned based on prior reports.25 (link)
