Mouse fetal livers were extracted from E14.5 embryos homozygous for the floxed LDB1 gene with or without CRE under control of the Mx1 gene promoter (Li et al. 2010 (link)). Cells were cultured as described (von Lindern et al. 2001 (link)). CRE expression was induced by 250 U/mL IFN-β (Millipore) in culture medium. Genotyping and Cre-mediated deletion were as described (Li et al. 2010 (link)).
Protocol detail
Conditional Genetic Manipulation of Fetal Mouse Liver
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Corresponding Organization :
Other organizations : National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
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Variable analysis
independent variables
- Cre expression under control of the Mx1 gene promoter
- Ldb1 gene deletion
- Mouse fetal livers extracted from E14.5 embryos homozygous for the floxed LDB1 gene
- Cell culture conditions as described in von Lindern et al. 2001
- Positive control: Mouse fetal livers from E14.5 embryos homozygous for the floxed LDB1 gene with Cre under control of the Mx1 gene promoter and treated with 250 U/mL IFN-β to induce Cre expression
- Negative control: Mouse fetal livers from E14.5 embryos homozygous for the floxed LDB1 gene without Cre under control of the Mx1 gene promoter
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