Dendritic Cell-Based Vaccine Against Measles Virus
Corresponding Organization :
Other organizations : Paul Ehrlich Institut, German Center for Infection Research, University of North Carolina at Chapel Hill
Protocol cited in 4 other protocols
Variable analysis
- Production of lentiviral vectors
- Generation of antigen-expressing dendritic cell lines
- Virus inoculation in animal experiments
- Virus neutralization test
- Plaque reduction neutralization test
- IFN-γ ELISpot analysis
- T cell proliferation assay
- CTL killing assay
- Virus titers in organs of infected animals
- Cells
- Plasmids
- Viruses
- MeV genome sequence analysis
- NGS library preparation and sequencing
- RNA sequence analysis
- Immunoperoxidase monolayer assay
- Western blot analysis
- Total IgG and IgG1-/IgG2a quantification
- Th1/Th2 cytokine multiplex assay
- RNA preparation
- Quantitative RT-PCR
- Statistical analyses
- Positive controls
- Negative controls
Annotations
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