Dendritic Cell-Based Vaccine Against Measles Virus

Detailed descriptions of the materials, methods, and equipment used in this work, including cells, plasmids, production of lentiviral vectors and generation of antigen-expressing dendritic cell lines, viruses, MeV genome sequence analysis, NGS library preparation and sequencing, RNA sequence analysis, immunoperoxidase monolayer assay, Western blot analysis, animal experiments, total IgG and IgG1-/IgG2a quantification, Th1/Th2 cytokine multiplex assay, virus neutralization test, plaque reduction neutralization test, IFN-γ ELISpot analysis, ICS, T cell proliferation assay, CTL killing assay, virus titers in organs of infected animals, RNA preparation, quantitative RT-PCR, and statistical analyses, are provided in SI Appendix, Supplementary Extended Materials and Methods.