Protocol detail

Western Blotting Antibody Validation

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Western blotting was carried out as described previously ^{49 (link),50 (link)}. Primary antibodies included antibodies against Twist1 (ab50887), Foxa1 (ab23738), c-Fos (ab53036, Abcam, Cambridge, MA), E-cadherin (610182, BD Bioscience, San Jose, CA), Vimentin (5741), c-Jun (9165), HA (3724, Cell Signaling, Danvers, MA) and β-actin (Sigma, St Louis, MO) and horseradish peroxidase (HRP) conjugated secondary antibody (Bio-rad, Hercules, CA) were used in the study.

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Xu Y., Qin L., Sun T., Wu H., He T., Yang Z., Mo Q., Liao L, & Xu J. (2016). Twist1 Promotes Breast Cancer Invasion and Metastasis by Silencing Foxa1 Expression. Oncogene, 36(8), 1157-1166.

Publication 2016

ab50887 ab23738 ab53036 E-cadherin Vimentin HA (3724, β-actin horseradish peroxidase (HRP) conjugated secondary antibody

Actin Antibodies Antibody C fos C jun E cadherin Foxa1 Horseradish peroxidase Twist1 Vimentin

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of Twist1, Foxa1, c-Fos, E-cadherin, Vimentin, c-Jun, and HA

control variables

None explicitly mentioned

controls

Positive controls: β-actin
Negative controls: Not specified

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