Canine MDCKII (ref. 26 (link)), porcine NSK and NPTr cells30 (link) and human A549 (ref. 31 (link)) and HEK293T cells32 (link) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM L -glutamine, 100 U penicillin per ml and 100 μg streptomycin per ml. Chicken hepatocellular epithelial cell line (LMH)33 (link) and chicken fibrolast cells (DF-1)34 (link) were grown in DMEM supplemented with 8% (v/v) FCS, 2% (v/v) chicken serum, 2 mM L -glutamine, 100 U penicillin per ml and 100 μg streptomycin per ml. Bat cells EpoNi/22.1 (ref. 35 (link)) and CarperAEC.B-3 (ref. 36 (link)) were maintained in DMEM supplemented with 10% (v/v) FCS, 2 mM L -glutamine, 100 U penicillin per ml, 100 μg streptomycin per ml, 1 mM sodium pyruvate and 0.1 mM non-essential amino acids (NEAAs, Cyagen catalogue number NEAA-10201-100). Primary HBAE cells (MucilAir, Epithelix Sàrl) reconstituted in Transwells of 6.5 mm of diameter with a pore size of 0.4 μm (Costar 3470, Corning) were maintained in MucilAir Culture Medium.
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Cell Culture Maintenance Protocols for Diverse Cell Lines
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Other organizations : Institute of Medical Microbiology and Hygiene, University Medical Center Freiburg, Icahn School of Medicine at Mount Sinai, Friedrich-Loeffler-Institut
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Variable analysis
independent variables
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- Cell lines used: Canine MDCKII, porcine NSK and NPTr, human A549 and HEK293T, chicken LMH and DF-1, bat EpoNi/22.1 and CarperAEC.B-3, primary HBAE cells
- Culture medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U penicillin per ml and 100 μg streptomycin per ml
- For chicken cells (LMH and DF-1): DMEM supplemented with 8% (v/v) FCS, 2% (v/v) chicken serum, 2 mM L-glutamine, 100 U penicillin per ml and 100 μg streptomycin per ml
- For bat cells (EpoNi/22.1 and CarperAEC.B-3): DMEM supplemented with 10% (v/v) FCS, 2 mM L-glutamine, 100 U penicillin per ml, 100 μg streptomycin per ml, 1 mM sodium pyruvate and 0.1 mM non-essential amino acids (NEAAs)
- For primary HBAE cells: MucilAir Culture Medium
- None explicitly mentioned
- None explicitly mentioned
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