EBV Detection via EBER ISH in Tissue

The status of EBV infection was assessed by ISH detection for EBER on paraffin-embedded tissue sections using fluorescein-labeled oligonucleotide probes (INFROM EBER Probe, Ventana), as previously described [11 (link)]. The visualization system used was the BenchMark XT with enzymatic digestion (ISH Protease 2, Ventana) and the iVIEW Blue v3 Detection Kit (Ventana). A known EBV-positive tumor was used as a positive control in each run. An appropriate negative control section was also included.

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Yu B.H., Zhang Y., Xue T., Shui R.H., Lu H.F., Zhou X.Y., Zhu X.Z, & Li X.Q. (2021). The clinicopathological relevance of uniform CD56 expression in anaplastic large cell lymphoma: a retrospective analysis of 18 cases. Diagnostic Pathology, 16, 1.

Publication 2021

INFROM EBER Probe BenchMark XT ISH Protease 2 iVIEW Blue v3 Detection Kit

Digestion Ebv infection Embedded paraffin Enzymatic Fluorescein Oligonucleotide probes Protease Tissue Tumor

Corresponding Organization :

Other organizations : Fudan University Shanghai Cancer Center

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Variable analysis

independent variables

ISH detection for EBER on paraffin-embedded tissue sections
Fluorescein-labeled oligonucleotide probes (INFROM EBER Probe, Ventana)

dependent variables

Status of EBV infection

control variables

Enzymatic digestion (ISH Protease 2, Ventana)
IVIEW Blue v3 Detection Kit (Ventana)

controls

Known EBV-positive tumor
Appropriate negative control section

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