Leaf and mixed stage inflorescence tissue were flash frozen in liquid nitrogen, and then the tissue was ground to a fine powder with a mortar and pestle. Leaf tissue was used for genomic and RNA-Seq, and the tissues used for each MethylC-Seq experiment is listed in Supplementary Table 1 . DNA was isolated using a Qiagen Plant DNeasy kit (Qiagen, Valencia, CA) following the manufacturer’s recommendations. RNA was isolated using the Qiagen Plant RNeasy kit (Qiagen) following the manufacturer’s instructions.
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Tissue Preparation and Nucleic Acid Extraction
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Corresponding Organization :
Other organizations : Salk Institute for Biological Studies, Scripps Research Institute
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Variable analysis
independent variables
- Tissue type (leaf vs. mixed stage inflorescence)
- Genomic DNA
- DNA methylation (from MethylC-Seq)
- Qiagen Plant DNeasy kit for DNA isolation
- Qiagen Plant RNeasy kit for RNA isolation
- No positive or negative controls were explicitly mentioned.
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