Immunohistochemical staining was performed as previously described (22 (link), 23 (link)). Briefly, 8 μm-thick sections were cut, dehydrated, and processed on a Bond III (Leica Biosystems Wetzlar, Germany). The ready-to-use (RTU) primary antibodies were from Novocastra (Leica Biosystems, Wetzlar, Germany), optimized for use on Bond III. The staining was performed with the following antibodies:
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