Co-immunoprecipitation of miRNP

Co-immunoprecipitation (Co-IP) of miRNP with anti-Ago1 (Abcam, Cambridge, MA, USA) or IgG (Sigma) was performed as previously described (Tan et al, 2009 (link)). RNA co-immunoprecipitated with anti-Ago1 or IgG antibodies was extracted using TRIzol LS (Invitrogen) as described in a previous publication (Cheng et al, 2008 (link)). Total RNA was subjected to DNA digestion as described above.

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Shi L., Zhang B., Sun X., Lu S., Liu Z., Liu Y., Li H., Wang L., Wang X, & Zhao C. (2014). MiR-204 inhibits human NSCLC metastasis through suppression of NUAK1. British Journal of Cancer, 111(12), 2316-2327.

Publication 2014

anti-Ago1 TRIzol LS

Co immunoprecipitation Digestion Igg antibodies Trizol

Corresponding Organization :

Other organizations : Weifang Medical University

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Variable analysis

independent variables

Anti-Ago1 antibody (Abcam, Cambridge, MA, USA)
IgG antibody (Sigma)

dependent variables

RNA co-immunoprecipitated with anti-Ago1 or IgG antibodies

control variables

Co-immunoprecipitation (Co-IP) protocol
RNA extraction using TRIzol LS (Invitrogen)
DNA digestion

controls

Positive control: Co-IP with anti-Ago1 antibody
Negative control: Co-IP with IgG antibody

Annotations

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