Mouse Strains for Immunology Research

Female and male mice were bred and housed by the Biomedical Research Unit at the Malaghan Institute of Medical Research. All animal experiments were performed in accordance with relevant guidelines and regulations and were approved by Victoria University of Wellington animal ethics committee. Animals used included: C57BL/6J (originally from The Jackson Laboratory, Bar Harbor, ME, USA) and the CD45.1 congenic strain B6.SJL-Ptprca Pepc^b/BoyJ (from Ozgene Pty, Bentley, WA, Australia), BALB/cJ mice (The Jackson Laboratory), Cd1d^−/− mice^{33 (link)} and Traj18^−/− mice (B6(Cg)-Traj18^tm1.1Kro/J; The Jackson Laboratory).^{34 (link)}Ifnar1^flox/flox mice^{35 (link)} were crossed with CD11c-cre mice (Tg(Itgax-cre,-EGFP)4097Ach), and then an F2 cross performed to give cre-positive Ifnar1^ΔCD11c mice and cre-negative littermates used as controls (Ifnar1^+/+). Experiments in Clec9a-DTR mice, which express the human diphtheria toxin (DT) receptor under the control of the Clec9a promoter,^{36 (link)} and Siglec-H-DTR mice which express the human DT receptor under the control of the Siglec-H promoter,^{36 (link)} were conducted in F1 crosses with C57BL/6J mice. Both were supplied by Nanyang Technological Unit, Singapore.