The following serological tests were used to check the serum samples from the dogs for the presence of antibodies specific to the rough and smooth Brucellae: The RSAT, the 2-ME TAT, rose Bengal test (RBT), and the buffered acidified plate antigen test (BAPAT). DNA of Brucella canis and other smooth Brucellae were identified in buffy coat samples separated from whole blood collected from seropositive dogs by three types of conventional PCRs (AMOS-PCR, Bruce-ladder PCR, and BcSS-PCR).
Brucella canis rough antigen that was used in the RSAT and 2-ME TAT was prepared from heat-killed cells of B. canis reference strain RM666 suspended in a formalized phosphate-buffered saline solution. Brucella canis 2-ME TAT antigen was obtained from the National Veterinary Services Laboratory (NVSL) Ames IA 50010, USA. Both RSAT and 2-ME TAT were carried out in this study to detect antibodies against B. canis, according to the technique adopted by Alton et al. [1 ].
RBT and BAPAT originating from smooth Brucellaabortus antigens were performed in this study to test dog blood samples for the detection of smooth Brucella antibodies according to the techniques described by Alton et al. [1 ] and World Organization for Animal Health (WOAH) [2 ].
Brucella canis rough antigen that was used in the RSAT and 2-ME TAT was prepared from heat-killed cells of B. canis reference strain RM666 suspended in a formalized phosphate-buffered saline solution. Brucella canis 2-ME TAT antigen was obtained from the National Veterinary Services Laboratory (NVSL) Ames IA 50010, USA. Both RSAT and 2-ME TAT were carried out in this study to detect antibodies against B. canis, according to the technique adopted by Alton et al. [1 ].
RBT and BAPAT originating from smooth Brucellaabortus antigens were performed in this study to test dog blood samples for the detection of smooth Brucella antibodies according to the techniques described by Alton et al. [1 ] and World Organization for Animal Health (WOAH) [2 ].
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