Human TREM2/DAP12 is overexpressed in HEK293 cell line (RRID: CVCL_0045, ATCC: PTA-4488) by transfection of a dual promoter pBudCE4.1 vector driving expression of TREM2 under the CMV promoter and DAP12 the under the EF1a promoter. Stable clones were isolated after zeocin selection (800 µg ml−1 for 10 days), and TREM2 was detected by flow cytometry (APC-conjugated rat anti-human/mouse-TREM2 monoclonal antibody, R&D Systems, MAB17291). The clone showing the highest TREM2 expression level was selected.
van Lengerich B., Zhan L., Xia D., Chan D., Joy D., Park J.I., Tatarakis D., Calvert M., Hummel S., Lianoglou S., Pizzo M.E., Prorok R., Thomsen E., Bartos L.M., Beumers P., Capell A., Davis S.S., de Weerd L., Dugas J.C., Duque J., Earr T., Gadkar K., Giese T., Gill A., Gnörich J., Ha C., Kannuswamy M., Kim D.J., Kunte S.T., Kunze L.H., Lac D., Lechtenberg K., Leung A.W., Liang C.C., Lopez I., McQuade P., Modi A., Torres V.O., Nguyen H.N., Pesämaa I., Propson N., Reich M., Robles-Colmenares Y., Schlepckow K., Slemann L., Solanoy H., Suh J.H., Thorne R.G., Vieira C., Wind-Mark K., Xiong K., Zuchero Y.J., Diaz D., Dennis M.S., Huang F., Scearce-Levie K., Watts R.J., Haass C., Lewcock J.W., Di Paolo G., Brendel M., Sanchez P.E, & Monroe K.M. (2023). A TREM2-activating antibody with a blood–brain barrier transport vehicle enhances microglial metabolism in Alzheimer’s disease models. Nature Neuroscience, 26(3), 416-429.
Anti antibody CloneFlow cytometry Hek293 cell line Human Monoclonal antibody Mouse Transfection vector Trem2Zeocin
Corresponding Organization :
Other organizations :
Denali Therapeutics (United States), German Center for Neurodegenerative Diseases, Ludwig-Maximilians-Universität München, Takeda (United States)
Transfection of a dual promoter pBudCE4.1 vector driving expression of TREM2 under the CMV promoter and DAP12 under the EF1a promoter
dependent variables
TREM2 expression level detected by flow cytometry (APC-conjugated rat anti-human/mouse-TREM2 monoclonal antibody, R&D Systems, MAB17291)
control variables
Zeocin selection (800 µg ml^-1 for 10 days)
controls
Positive control: N/A
Negative control: N/A
Annotations
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