Characterizing lincRNA-EPS Interactions

The RIP Kit (Millipore) was used to analyze the interaction of lincRNA-EPS with HNRNPL and MYH6 in HL-1 cells [25 (link)]. In brief, HL-1 cells were suspended in 200  μL of lysis buffer. Anti-IgG and anti-HNRNPL antibodies were used to pre-treat the magnetic beads, which were used to immunoprecipitate the cell suspension overnight at 4°C. The RNAs were purified from the protein-RNA complex and analyzed by qPCR using lincRNA-EPS and MYH6 primers.

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Zhang H., Kou X., Xiao D, & Yu Z. (2023). Long non-coding RNA lincRNA-erythroid prosurvival attenuates inflammation by enhancing myosin heavy chain 6 stability through recruitment of heterogeneous nuclear ribonucleoprotein L in myocardial infarction. Bioengineered, 13(6), 14426-14437.

Publication 2022

RIP Kit

Anti antibodies Anti igg Buffer Lincrna Primers Protein

Corresponding Organization : Jinan Central Hospital

Other organizations : Shandong Provincial Hospital

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Variable analysis

independent variables

Anti-IgG antibody
Anti-HNRNPL antibody

dependent variables

Interaction of lincRNA-EPS with HNRNPL
Interaction of lincRNA-EPS with MYH6

control variables

HL-1 cells
Lysis buffer
Magnetic beads
Protein-RNA complex
QPCR using lincRNA-EPS and MYH6 primers

controls

Positive control: Anti-HNRNPL antibody
Negative control: Anti-IgG antibody

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