Protocol detail

Culturing Human Cancer and Bronchial Cell Lines

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The human cancer cell lines (H1299 (Cat No. FH0908), A549 (Cat No. FH0045), H292 (Cat No. FH0085) obtained from FuHeng Biology (Shanghai, China); SW900 (Cat No. BNCC275987) obtained from BNBIO (Beijing, China); PC9 (Cat No. MZ‐2682) obtained from Mingzhou Bio), were cultured as previously reported.17 (link), 18 (link) The human bronchial epithelial cell lines (HBE; BNBIO, Cat No. BNCC338600) and BEAS‐2b (FuHeng Biology, Cat No. FH0319) were cultured in Dulbecco's modified eagle medium (DMEM) purchased from HyClone Laboratories Inc. supplemented with 5% foetal bovine serum and 1% penicillin/streptomycin solution, at 37°C in a humidified atmosphere containing 5% CO₂.

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Li L., Li Z., Qu J., Wei X., Suo F., Xu J., Liu X., Chen C, & Zheng S. (2022). Novel long non‐coding RNA CYB561‐5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non‐small cell lung cancer. Journal of Cellular and Molecular Medicine, 26(5), 1402-1412.

Publication 2022

Dulbecco's modified eagle medium (DMEM)

Atmosphere Bronchial Cancer Cell lines Cultured medium Eagle Foetal bovine serum Human Penicillin Streptomycin

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Variable analysis

independent variables

Human cancer cell lines (H1299, A549, H292, SW900, PC9)
Human bronchial epithelial cell lines (HBE, BEAS-2b)

dependent variables

Not explicitly mentioned

control variables

Cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 5% foetal bovine serum and 1% penicillin/streptomycin solution
Cells were cultured at 37°C in a humidified atmosphere containing 5% CO2

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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