IFNγ Production Assay for T Cells

IFNγ was measured as described from BDC2.5 cells 4h after 500μg i.v. of acetylated p31 peptide (YVRPLWVRME) (Genemed Sythesis) or OT-I cells after 1μg/ml SIINFEKL ex vivo for 4h (New England Peptide) [10 (link), 11 (link)].

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Pauken K.E., Nelson C.E., Martinov T., Spanier J.A., Heffernan J.R., Sahli N.L., Quarnstrom C.F., Osum K.C., Schenkel J.M., Jenkins M.K., Blazar B.R., Vezys V, & Fife B.T. (2015). Identification of autoreactive CD4+ and CD8+ T cell subsets resistant to PD-1 pathway blockade. Journal of immunology (Baltimore, Md. : 1950), 194(8), 3551-3555.

Publication 2015

SIINFEKL

Cells Ifnγ Peptide

Corresponding Organization : University of Minnesota

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Variable analysis

independent variables

Administration of 500μg i.v. of acetylated p31 peptide (YVRPLWVRME) to BDC2.5 cells
Stimulation of OT-I cells with 1μg/ml SIINFEKL ex vivo

dependent variables

Measurement of IFNγ from BDC2.5 cells 4h after peptide administration
Measurement of IFNγ from OT-I cells after 1μg/ml SIINFEKL stimulation for 4h

control variables

Time point of 4h for IFNγ measurement in both cell types

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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