sNfL of 112 patients with CIS and MS and of 62 healthy donors was measured by SiMoA technology as previously described.7 (link) Briefly, blood samples were spun at 2000g at room temperature for 10 minutes within 2 hours after withdrawal and stored in polypropylene tubes at −80°C. Serum NfL was measured by SiMoA HD-1 (Quanterix) using the NF-Light Advantage Kit (Quanterix) according to the manufacturer's instructions. Samples were measured in duplicates, and the intra-assay coefficient of variation of all samples was 4.5%. sNfL measurements were performed in a blinded fashion without information about clinical data.
Protocol detail
Serum Neurofilament Light Chain Quantification
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Other organizations : University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz
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Variable analysis
independent variables
- None explicitly mentioned
- SNfL (serum neurofilament light chain)
- Blood sample processing (spun at 2000g at room temperature for 10 minutes within 2 hours after withdrawal)
- Blood sample storage (stored in polypropylene tubes at −80°C)
- SNfL measurement (measured by SiMoA HD-1 (Quanterix) using the NF-Light Advantage Kit (Quanterix) according to the manufacturer's instructions)
- Sample replication (samples were measured in duplicates)
- Blinding (sNfL measurements were performed in a blinded fashion without information about clinical data)
- Positive control: None explicitly mentioned
- Negative control: None explicitly mentioned
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