Monocyte-Derived Macrophage Isolation
Corresponding Organization :
Other organizations : Emory University, Kyung Hee University, Albert Einstein College of Medicine, Veterans Health Administration
Protocol cited in 1 other protocol
Variable analysis
- Positive selection using MACS CD14+ beads to isolate primary human monocytes from peripheral blood buffy coats
- Maturation of monocytes into monocyte-derived macrophages (MDMs) in the presence of 5 ng/ml hGM-CSF (Miltenyl Biotec) treated at days 0 and 2 of maturation
- Outcomes or properties of the monocyte-derived macrophages (MDMs) at day 7 of maturation
- Isolation of primary human monocytes from peripheral blood buffy coats using the previously described method [50]
- Maturation duration of monocytes into MDMs (7 days)
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
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