Organoids were derived using the human Tumor Dissociation Kit (Miltenyi Biotec) from the ER+ patient derived xenografts from the Breast Cancer Genome Guided Therapy Study according to a previously described protocol (15 (link)). Organoids were cultured in 96-well low binding NanoCulture plate (Organogenix) in DMEM supplemented with 10% FCS, 1% glutamax, 1% sodium pyruvate, non-essential amino acids, and 1% Penicillin-Streptomycin (Life Technologies) at 37°C, 5% CO2. Organoid growth was measured as previously described (15 (link)) using the CellTiter-Glo Viability assay (Promega).
Protocol detail
Estrogen Receptor+ Breast Cancer Organoid Culture
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Assay
Breast cancer
Essential amino acids
Genome
Human
Organoid
Patient
Penicillin
Promega
Pyruvate
Sodium
Streptomycin
Therapy
Tumor
Xenografts
Top 5 similar protocols
Variable analysis
independent variables
- Tumor Dissociation Kit (Miltenyi Biotec)
- Organoid growth
- DMEM supplemented with 10% FCS, 1% glutamax, 1% sodium pyruvate, non-essential amino acids, and 1% Penicillin-Streptomycin
- Culture conditions (37°C, 5% CO2)
- Not specified
- Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!