Anti-human PD-L1/CD274, -PE and –APC (Clone# 29E.2A3, catalog#329745, 329706, 329708), anti-FLAG antibody (Clone# L5, catalog#637304), Alexa Fluor 555 goat anti-rat IgG antibody (Catalog#405420), Alexa Fluor 467 goat anti-rat IgG antibody(Catalog#405416), human IFNγ (Catalog#570208), human IFNα (Catalog#592704), human IL-2 (Catalog#589104), and Zombie UV dye (Catalog#77474) were purchased from Biolegend (San Diego, CA, USA). Human IFNβ (Catalog#8499-1F) was purchased from R&D Systems (Minneapolis, MN 55413). Heat Inactivated human AB serum was purchased from Innovative Research, Inc (Catalog# IPLA-SERAB-27146, MI 48377, USA). Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation was purchased from ThermoFisher Scientific (Catalog# 11131D, Waltham, MA, USA). The CF33-hNIS-Δ (CF33-hNIS-ΔF14.5L) virus [24 (link), 25 (link)], CF33-GFP [ref.26 (link)], and the CF33-hNIS-antiPDL1 virus [23 (link)] were generated in our lab as previously described. Anti-sodium/Iodide Symporter (hNIS) monoclonal antibody was purchased from EMD Millipore Corp (Catalog# MAB3564, Billerica, MA, USA). Goat anti-rabbit IgG (H+L) Alexa Fluor 555 antibody (Catalog# A21434) and Goat anti-mouse Alexa Fluor 488 antibody (Catalog# A11029) were purchased from Invitrogen Corporation (Carlsbad, CA, USA).