Western blotting was performed as previously described30 (link), 34 (link). A list of primary and secondary antibodies, including manufacturer number and working concentration, may be found in Supplementary Table 1 . Immunoprecipitation to detect activated RhoA was performed using a pulldown assay kit (Cytoskeleton, Inc.) according to manufacturer instructions.
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Detecting Activated RhoA by Western Blotting
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Corresponding Organization : University of Rochester
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Variable analysis
independent variables
- Activated RhoA
- Activated RhoA
- Western blotting procedure as previously described in references 30 and 34
- Primary and secondary antibodies used, including manufacturer number and working concentration, as listed in Supplementary Table 1
- Positive control: Pulldown assay kit (Cytoskeleton, Inc.) used to detect activated RhoA
- Negative control: Not explicitly mentioned
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