Apoptosis Detection in Fibroblast-Like Synoviocytes

RA-FLSs cell apoptosis in different experimental groups was measured by Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit (BD Biosciences, San Jose, CA) according to the manufacturer’s instructions [28 (link)]. FLSs cells in the logarithmic growth phase were collected and inoculated into a six-well plate. Then, 5 μL Annexin V-FITC and PI were added to the sample for 15 min in the dark and thus detected by flow cytometer (BD, San Jose, CA, USA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Xu W., Liu X., Qu W., Wang X., Su H., Li W, & Cheng Y. (2023). Exosomes derived from fibrinogen-like protein 1-overexpressing bone marrow-derived mesenchymal stem cells ameliorates rheumatoid arthritis. Bioengineered, 13(6), 14545-14561.

Publication 2022

Annexin V-fluorescein FITC)/propidium iodide (PI) apoptosis detection kit

Annexin v Apoptosis Cells Fluorescein Isothiocyanate Propidium iodide

Corresponding Organization : Yantaishan Hospital

Other organizations : Yuhuangding Hospital, Qingdao University

Top 5 similar protocols

Variable analysis

independent variables

Experimental groups

dependent variables

RA-FLSs cell apoptosis

control variables

FLSs cells in the logarithmic growth phase

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!