We isolated PBMCs, challenged them ex vivo, and measured the cytokines and chemokines that were produced by the cells [46 (link), 47 (link)].
We conducted two studies: a “mycotoxin study” where the challenges were either a mycotoxin (SG), an aqueous S. chartarum extract with a mix of mycotoxins and other antigens (SST), a non-specific toxin (ionomycin [positive control, VWR, Radnor, PA]), or media alone (negative control), each with or without the adjuvant phorbol 12-myristate 13-acetate (PMA, VWR, Radnor, PA); and a “mold spore study” where the challenges were with intact mold spores, a non-specific toxin (phytohemagglutinin [PHA]), or media alone.
We conducted two studies: a “mycotoxin study” where the challenges were either a mycotoxin (SG), an aqueous S. chartarum extract with a mix of mycotoxins and other antigens (SST), a non-specific toxin (ionomycin [positive control, VWR, Radnor, PA]), or media alone (negative control), each with or without the adjuvant phorbol 12-myristate 13-acetate (PMA, VWR, Radnor, PA); and a “mold spore study” where the challenges were with intact mold spores, a non-specific toxin (phytohemagglutinin [PHA]), or media alone.
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