Folates in tissue samples and erythrocytes were determined by means of LC-MS/MS (Finnigan Surveyor Plus high performance liquid chromatography (HPLC) System, Thermo Electron Corporation, Waltham, MA, USA; triple quadrupole TSQ quantum discovery mass spectrometer, Thermo Electron Corporation, Waltham, MA, USA). The vitamers were separated on a YMC Pack Pro C18 column (150 × 3 mm, 3 µm, YMC, Kyoto, Japan). The mobile phase for gradient elution consisted of 0.1% (v/v) aqueous formic acid (eluent A) and acetonitrile containing 0.1% (v/v) formic acid (eluent B) at a flow rate of 0.3 mL/min.
The system for Hcy, AdoMet, and AdoHcy measurement consisted of a Shimadzu Prominence LC-20A System (Shimadzu, Kyoto, Japan) and an API 4000 Q-Trap mass spectrometer (AB Sciex, Foster City, CA, USA). Analyte separation was carried out on a Phenomenex Gemini reversed phase column (110A 3u, 150 × 4.60 mm, Phenomenex, Aschaffenburg, Germany). The mobile phase for gradient elution consisted of 0.1% (v/v) aqueous formic acid (eluent A) and acetonitrile containing 0.1% (v/v) formic acid (eluent B) at a flow of 0.4 mL/min.
Gradients and source parameters of the LC-MS instrument for tissue, plasma samples [19 (link)], and erythrocytes [29 (link)] have been published earlier.
The system for Hcy, AdoMet, and AdoHcy measurement consisted of a Shimadzu Prominence LC-20A System (Shimadzu, Kyoto, Japan) and an API 4000 Q-Trap mass spectrometer (AB Sciex, Foster City, CA, USA). Analyte separation was carried out on a Phenomenex Gemini reversed phase column (110A 3u, 150 × 4.60 mm, Phenomenex, Aschaffenburg, Germany). The mobile phase for gradient elution consisted of 0.1% (v/v) aqueous formic acid (eluent A) and acetonitrile containing 0.1% (v/v) formic acid (eluent B) at a flow of 0.4 mL/min.
Gradients and source parameters of the LC-MS instrument for tissue, plasma samples [19 (link)], and erythrocytes [29 (link)] have been published earlier.
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