Coleorhiza samples were collected by sterile Fisher brand razor blades. To remove any residual moisture, coleorhizae were lyophilized overnight in the freeze dryer prior to samples preparation for FTIR spectroscopy. Dried samples were then grinded by mortar and pestle to provide a homogeneous mixture. To prepare a potassium bromide (KBr) pellet or disk, each sample was added to a concentration rate of 1.3%, grinded with a mortar and pestle to a fine powder. The fine powder of sample/KBr mixture was transferred on the dye set and made into a pellet in triplicate under 7 psi (pound-force per square inch) for 2 minutes, using a hydraulic press (Manual hydraulic press 15 Ton, Specac, Orpington, UK). Pellets (12 mm) were transferred on clean sample dishes for FTIR measurement22 (link).
FTIR spectra were collected using the Bruker IFS 66 V/S spectrometer with a KBr beam splitter at the mid infrared beamline using globar (silicon carbide) as the infrared source. Each IR spectrum was measured and recorded at resolution of 2 cm−1 and at sample scan time of 64 in the range of 4000–600 cm−1 22 (link). The same sample was repeatedly scanned three times for spectral data collection.
FTIR spectra were collected using the Bruker IFS 66 V/S spectrometer with a KBr beam splitter at the mid infrared beamline using globar (silicon carbide) as the infrared source. Each IR spectrum was measured and recorded at resolution of 2 cm−1 and at sample scan time of 64 in the range of 4000–600 cm−1 22 (link). The same sample was repeatedly scanned three times for spectral data collection.
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