Viral DNA was extracted from tissue or blood samples using MagMax Core kit and MagMax96 extractor (Thermo Fisher) following the manufacturer’s instructions; samples were stored at −20 °C until further analysis. All the samples were screened for the presence of PCV3, PCV2, and PPV by qualitative real-time PCR, as previously described [44 (link),45 (link),46 (link)]. Samples with Ct value of less than 40 were considered positive.
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