Drosophila Genetic Background Preparation

All flies were maintained on a standard cornmeal/molasses diet at 25 °C and 75% humidity on a 12-hour day and 12-hour night cycle. The following lines were acquired from the Bloomington Stock Center: lid¹⁰⁴²⁴ (BL# 12367) and lid^k06801 (BL# 10403). PSR^FM1 was provided by Kristin White (Massachusetts General Hospital, Charlestown, MA). The generation and verification of the knockout lines and tagged transgenes is described in Shalaby et al.^{32 (link)}. Flies were outcrossed in groups of >20 for at least 5 generations to the w* Berlin genetic background for behavioral analyses.

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Shalaby N.A., Pinzon J.H., Narayanan A.S., Jin E.J., Ritz M.P., Dove R.J., Wolfenberg H., Rodan A.R., Buszczak M, & Rothenfluh A. (2018). JmjC domain proteins modulate circadian behaviors and sleep in Drosophila. Scientific Reports, 8, 815.

Publication 2018

lid10424 lidk06801

Diet Flies Genetic background Humidity Molasses Transgenes

Corresponding Organization :

Other organizations : The University of Texas Southwestern Medical Center, Freie Universität Berlin, University of Utah

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Variable analysis

independent variables

lid¹⁰⁴²⁴
lid^k06801
PSR^FM1

dependent variables

Not explicitly mentioned

control variables

Standard cornmeal/molasses diet
Temperature: 25 °C
Humidity: 75%
Light cycle: 12-hour day and 12-hour night
w* Berlin genetic background

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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