Stem Cell Derivation and Characterization

Endothelial cells and mesenchymal cells were derived as described previously^{15 (link)}. iPSC-NSC were derived as described previously^{31 (link)}. Gene expression was evaluated using qPCR and flow cytometry. Following antibodies were used for flow cytometric analysis: PE-conjugated mouse anti-human CD140b (PDGFRB; 558821, BD Biosciences); APC-conjugated anti-human CD166 (ALCAM; 130-119-809, Miltenyi Biotec); anti- human CD105, human CD73, and human CD90 (MSC phenotyping kit; 130-095-198, Miltenyi Biotec); FITC-conjugated mouse anti-human CD31 (557508, BD Biosciences); PE-conjugated mouse anti-human CD144 (561714, BD Biosciences).

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Sekine K., Tsuzuki S., Yasui R., Kobayashi T., Ikeda K., Hamada Y., Kanai E., Camp J.G., Treutlein B., Ueno Y., Okamoto S, & Taniguchi H. (2020). Robust detection of undifferentiated iPSC among differentiated cells. Scientific Reports, 10, 10293.

Publication 2020

PE-conjugated mouse anti-human CD140b (PDGFRB; APC-conjugated anti-human CD166 (ALCAM; FITC-conjugated mouse anti-human CD31 PE-conjugated mouse anti-human CD144

Alcam Antibodies Cd73 Cd90 Endothelial cells Fitc Flow cytometric Gene expression Human Ipsc Mesenchymal cells Mouse

Corresponding Organization : The University of Tokyo

Other organizations : Yokohama City University, Institute of Molecular and Clinical Ophthalmology Basel, ETH Zurich

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Variable analysis

independent variables

Endothelial cells and mesenchymal cells derived as described previously
IPSC-NSC derived as described previously

dependent variables

Gene expression evaluated using qPCR and flow cytometry

control variables

No control variables explicitly mentioned

controls

No positive or negative controls explicitly mentioned

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