Rhodamine-Phalloidin-conjugate Staining of PAV-1 Cells

A Rhodamine-Phalloidin-conjugate (#R415, Thermo Fisher Scientific) was used to stain the microfilaments in PAV-1 cells according to a previously established protocol [19 (link),35 (link)]. The nuclei were counterstained by incubation in a 4′,6-diamidino-2-phenylindole (DAPI) solution (#D1306, Thermo Fisher Scientific), and the glass coverslips were mounted with PermaFluor aqueous mounting medium (#TA-030-FM, Thermo Fisher Scientific). Images of stained cells were captured using a Nikon Eclipse E80i fluorescence microscope (Nikon Europe, Düsseldorf, Germany) equipped with the NIS-Elements Vis software (Nikon Europe, version 3.22.01).

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Gäberlein K., Schröder S.K., Nanda I., Steinlein C., Haaf T., Buhl E.M., Sauvant P., Sapin V., Abergel A, & Weiskirchen R. (2023). Genetic Characterization of Rat Hepatic Stellate Cell Line PAV-1. Cells, 12(12), 1603.

Publication 2023

Rhodamine-Phalloidin-conjugate PermaFluor aqueous mounting medium Nikon Eclipse E80i fluorescence microscope NIS-Elements Vis software

Cells Fluorescence microscope Microfilaments Nuclei Rhodamine phalloidin

Corresponding Organization : Universitätsklinikum Aachen

Other organizations : University of Würzburg, Université de Bordeaux, Centre National de la Recherche Scientifique, École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine, Université Clermont Auvergne, Genetique Reproduction and Developpement, Centre Hospitalier Universitaire de Clermont-Ferrand

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Variable analysis

independent variables

Rhodamine-Phalloidin-conjugate (#R415, Thermo Fisher Scientific) used to stain the microfilaments in PAV-1 cells

dependent variables

Microfilament staining in PAV-1 cells

control variables

Previously established protocol [19 (link),35 (link)]
Incubation in a 4′,6-diamidino-2-phenylindole (DAPI) solution (#D1306, Thermo Fisher Scientific)
Mounting with PermaFluor aqueous mounting medium (#TA-030-FM, Thermo Fisher Scientific)
Nikon Eclipse E80i fluorescence microscope (Nikon Europe, Düsseldorf, Germany) equipped with the NIS-Elements Vis software (Nikon Europe, version 3.22.01)

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